Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Feb 6;6(3):402–406. doi: 10.1242/bio.021725

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 1. — Pro-cerebellar signaling molecules induce complex 3D organoid structures. (A) Timeline of protocol indicating what day specific factors were added to culture medium. For FGF2 arrows refer to lower (4 ng ml⁻¹, down arrow) and higher (20 ng ml⁻¹, up arrow) concentrations. (B) Bright-field images show typical examples of cultures at: d0 (as ESC colonies), d2 (EBs), d14 (after induction with RA+FGF8), d28 [after patterning/before maturation, with aggregates (indicated by numbers 1-5) varying in size and complexity], d28 (a large aggregate with complex features identified by letters a-c), d35 (same aggregate from d28 with same features indicated by letters a-c allowing comparison after 7 day maturation step with SAG). Scale bars: 100 μm.