Table 1.
The grouping of experimental rats, the dosage of GBRE supplementation and study period.
| No | Group (G) | Week 1 | Week 2, 3 | Week 4–12 |
|---|---|---|---|---|
| 1 | C (G1) | ND & NDW | ND & NDW | ND & NDW |
| 2 | LD (G2) | 500 mg/kg BW. of GBRE, ND & NDW | Buffer injection (i.p.) *, ND & NDW | 500 mg/kg BW. of GBRE, ND & NDW |
| 3 | HD (G3) | 1000 mg/kg BW. of GBRE, ND & NDW | Buffer injection (i.p.) *, ND & NDW | 1000 mg/kg BW. of GBRE, ND & NDW |
| 4 | PreLD (G4) | 500 mg/kg BW. of GBRE, ND & NDW | STZ injection (i.p.) *, ND & NDW | 500 mg/kg BW. of GBRE, ND & NDW |
| 5 | PreHD (G5) | 1000 mg/kg BW. of GBRE, ND & NDW | STZ injection (i.p.) *, ND & NDW | 1000 mg/kg BW. of GBRE, ND & NDW |
| 6 | DM (G6) | ND & NDW | STZ injection (i.p.) *, ND & NDW | ND & NDW |
| 7 | PostLD (G7) | ND & NDW | STZ injection (i.p.) *, ND & NDW | 500 mg/kg BW. of GBRE, ND & NDW |
| 8 | PostHD (G8) | ND & NDW | STZ injection (i.p.) *, ND & NDW | 1000 mg/kg BW. of GBRE, ND & NDW |
| 9 | Drug (G9) | ND & NDW | STZ injection (i.p.) *, ND & NDW | 50 mg/kg BW. of MFN, ND & NDW |
Note: C: control, LD: low dose, HD: high dose, PreLD: pre-treatment with low dose of GBRE, PreHD: pre-treatment with high dose of GBRE, PostLD: post-treatment with lose dose of GBRE, PostHD: post-treatment with high dose of GBRE, Drug: metformin, ND: normal diet, NDW: normal drinking water, DM: Diabetes Mellitus, GBRE: germinated black rice extract, i.p.: intraperitoneal injection, STZ: streptozotocin (45 mg/kg BW.), MFN: metformin (50 mg/kg BW.), *: The initial day of week 2 GBRE and the drug were supplemented to rat along with normal diet.