Figure 1.

C3H/10T1/2 cells were differentiated for 12 days using IBMX, dexamethasone and insulin. (a) Bright field images showing the differentiation status at day 12 of differentiation. Control cells (without differentiation mix) were compared to cells differentiated with the mix. Lipid droplets were stained red with Oil Red O. Magnification 200×; (b,e) Cells were harvested at given time points during differentiation and protein expression was detected with specific antibodies using a Western Blot approach. GAPDH was used as loading control; (c) Quantification of the protein expression of CK2α, CK2α’, and CK2β normalized to GAPDH; (d) CK2 activity during adipogenic differentiation of C3H/10T1/2 cells over time shown by the incorporation of [³²P]phosphate into the CK2 specific substrate peptide RRRDDDSDDD.