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C3H/10T1/2 cells were differentiated in the presence of DMSO (D) or 15 µM CX-4945 (CX) and harvested at given time points during differentiation. Proteins were extracted and separated on a 12.5% SDS-polyacrylamide gel and transferred to a PVDF-membrane. C/EBPβ, C/EBPδ, C/EBPα, and PPARγ2 were detected with specific antibodies. GAPDH was used as a loading control.
