Figure 1.

GL261 cells viability after treatment; (A) GL261 cells viability (%) “XTT assay” after 72 h of treatment with temozolomide (TMZ): 0 µM, 0.8 µM, 4 µM, 20 µM, 100 µM, 200 µM, 500 µM, 1000 µM, 5000 µM and 10,000 µM, apigenin (APG) and tetrabromobenzotriazole (TBB): 0 µM, 0.8 µM, 4 µM, 20 µM, 100 µM, 200 µM and 500 µM, CX-4945 and tetrabromo-deoxyribofuranosyl-benzimidazole (TDB): 0 µM, 0.2 µM, 2 µM, 5 µM, 20 µM, 50 µM, 100 µM, 200 µM and 500 µM); 100% cell viability was assigned to control cells treated with 0.8% dimethyl sulfoxide (DMSO) (v/v). Experiments were performed with n = 3–9, and mean ± SD values are shown; (B) EC₅₀ (Half maximal, 50% viability decrease, effective concentration) mean ± SD values obtained with the different treatments to GL261 cells after 72 h of incubation with the drug; (C) Boxplot of GL261 cells viability after TMZ and CX-4945 treatment. GL261 cells viability (%) “MTT assay” after 72 h of treatment. On the left side, control (n = 4), CX-4945 30 µM (n = 4), TMZ 1 mM (n = 4) and CX-4945 30 µM plus TMZ 1 mM (n = 3); on the right side, control (n = 4), CX-4945 50 µM (n = 4), TMZ 1.5 mM (n = 4) and CX-4945 50 µM and TMZ 1.5 mM (n = 3); 100% cell viability was assigned to control cells treated with 0.8% DMSO (v/v). As both experiments were performed at the same time, controls were acquired only once and, accordingly, the same control cells results are shown for both experimental conditions. Experiments were performed with n = 3–4, and mean ± SD values are shown. Boxplot (the limits of the box represent quartiles 1 (Q1) and 3 (Q3) of the distribution, the central line corresponds to the median (quartile 2). The whiskers symbolize the maximum and minimum values in each distribution.