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. 2016 Dec 30;49(3):151–159. doi: 10.1152/physiolgenomics.00120.2016

Table 3.

Description of the top-ranked DUBs from Table 2

Gene Symbol Ubiquitin Chain Specificity Cell Component Class Physiological Role
OTUB1 Lys-48 cytoplasm OTU Regulates RNF128-mediated ubiquitination but does not deubiquitinate polyubiquitinated RNF128; deubiquitinates estrogen receptor alpha (ESR1) (31); overexpression in mesangial cells enhanced the ubiquitination and degradation of decorin (40).
USP14 Lys-48 & Lys-63 proteasome USP Proteasome-associated deubiquitinase that releases ubiquitin from the proteasome targeted ubiquitinated proteins; ensures the regeneration of ubiquitin at the proteasome.; undergoes a reciprocal abundance change in the 200,000 g cytosolic fraction [down] and the 200,000 g pellet fraction [up] in mpkCCD cells in response to vasopressin (38); is a major regulator of the proteasome and one of three proteasome-associated deubiquitinating enzymes.
PSMD7 Lys-63 proteasome JAMM Acts as a regulatory subunit of the 26S proteasome.
PSMD14 Lys-63 proteasome JAMM Component of the 26S proteasome.; identified in or attached to the apical plasma membrane by surface biotinylation in cultured mouse mpkCCD cells (23).
USP7 Lys-48>Lys-63 endosome USP Undergoes a reciprocal abundance change in the 1,000 g pellet fraction [down] and the 200,000 g cytosolic fraction [up] in response to vasopressin (38).; protein has a half-life of 28.8 h in mouse mpkCCD cells (28); deubiquitylates and regulates FOXO4 transcriptional activity (36).
USP9X Lys-29; Lys-33 endosome, plasma membrane, cytosol USP Processing of both ubiquitin precursors and of ubiquitinated proteins.; prevents degradation of proteins through the removal of conjugated ubiquitin; vasopressin administration for 7 days increased mRNA abundance of Usp9x in mouse renal inner medulla (9).
OTUD4 Lys-48 endosome? OTU Undergoes a reciprocal abundance change in the 200,000 g pellet fraction [down] and the 17,000 g membrane fraction [up] in response to vasopressin (38); development role: interaction with Tsc22d3 (GILZ) to affect BMP signaling (35); GILZ positively regulates ENaC (29).
USP10 Lys-48 endosome USP Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling (4); Usp10 protein has a half-life of 26.2 h in mouse mpkCCD cells in the absence of vasopressin and a half-life of 29.3 h in presence of vasopressin (28).
UCHL5 Lys-48 proteasome UCH Associated with the 19S regulatory subunit of the 26S proteasome.

“Physiological Role” is an annotated function obtained from “[FUNCTION]” field of individual UniProt Protein records and the output of BIG (41).