Table 3.

Description of the top-ranked DUBs from Table 2

Gene Symbol	Ubiquitin Chain Specificity	Cell Component	Class	Physiological Role
OTUB1	Lys-48	cytoplasm	OTU	Regulates RNF128-mediated ubiquitination but does not deubiquitinate polyubiquitinated RNF128; deubiquitinates estrogen receptor alpha (ESR1) (31); overexpression in mesangial cells enhanced the ubiquitination and degradation of decorin (40).
USP14	Lys-48 & Lys-63	proteasome	USP	Proteasome-associated deubiquitinase that releases ubiquitin from the proteasome targeted ubiquitinated proteins; ensures the regeneration of ubiquitin at the proteasome.; undergoes a reciprocal abundance change in the 200,000 g cytosolic fraction [down] and the 200,000 g pellet fraction [up] in mpkCCD cells in response to vasopressin (38); is a major regulator of the proteasome and one of three proteasome-associated deubiquitinating enzymes.
PSMD7	Lys-63	proteasome	JAMM	Acts as a regulatory subunit of the 26S proteasome.
PSMD14	Lys-63	proteasome	JAMM	Component of the 26S proteasome.; identified in or attached to the apical plasma membrane by surface biotinylation in cultured mouse mpkCCD cells (23).
USP7	Lys-48>Lys-63	endosome	USP	Undergoes a reciprocal abundance change in the 1,000 g pellet fraction [down] and the 200,000 g cytosolic fraction [up] in response to vasopressin (38).; protein has a half-life of 28.8 h in mouse mpkCCD cells (28); deubiquitylates and regulates FOXO4 transcriptional activity (36).
USP9X	Lys-29; Lys-33	endosome, plasma membrane, cytosol	USP	Processing of both ubiquitin precursors and of ubiquitinated proteins.; prevents degradation of proteins through the removal of conjugated ubiquitin; vasopressin administration for 7 days increased mRNA abundance of Usp9x in mouse renal inner medulla (9).
OTUD4	Lys-48	endosome?	OTU	Undergoes a reciprocal abundance change in the 200,000 g pellet fraction [down] and the 17,000 g membrane fraction [up] in response to vasopressin (38); development role: interaction with Tsc22d3 (GILZ) to affect BMP signaling (35); GILZ positively regulates ENaC (29).
USP10	Lys-48	endosome	USP	Deubiquitinates CFTR in early endosomes, enhancing its endocytic recycling (4); Usp10 protein has a half-life of 26.2 h in mouse mpkCCD cells in the absence of vasopressin and a half-life of 29.3 h in presence of vasopressin (28).
UCHL5	Lys-48	proteasome	UCH	Associated with the 19S regulatory subunit of the 26S proteasome.

“Physiological Role” is an annotated function obtained from “[FUNCTION]” field of individual UniProt Protein records and the output of BIG (41).