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. 2017 Mar 31;7:45820. doi: 10.1038/srep45820

Figure 5. SiRNA-mediated knock down of FSTL1 promotes hypoxia-induced proliferation and migration of HPASMCs.

Figure 5

(a) QRT-PCR analysis of FSTL1 mRNA in HPASMCs transfected with siRNA or N.C., as normalized by GAPDH mRNA. n = 4. (b) Representative cropped western blots of FSTL1 protein in HPASMCs transfected with siRNA or N.C. n = 3. (c) Effect of FSTL1 siRNA transfection on cellular viability in MTT assay. n = 3. (d) Effect of FSTL1 siRNA transfection on DNA synthesis in BrdU assay for flow cytometer analysis. Cells in synthesis phrase (S, P4) at a cell cycle was calculated as the percent of P4/(P3 + P4 + P5). n = 3. (e) Effect of FSTL1 siRNA transfection on cellular migration in transwell chamber. Nuclei of trans-membrane cells were stained with DAPI (blue). n = 3. Bar = 50 μm. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. SiRNA = small interfering RNA. N.C. = negative control. P3 = G0/G1 phrase. P4 = S phase. P5 = G2 phrase. P6 = apoptosis phrase. N = normoxia. H = hypoxia. GAPDH = glyceraldehyde-3-phosphate dehydrogenase. DAPI = 4′, 6-diamidino-2-phenylindole. MTT = 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. BrdU = 5-bromo-2-deoxyuridine.