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. 2017 Mar 31;7:45820. doi: 10.1038/srep45820

Figure 7. FSTL1 inhibited hypoxic HPASMCs proliferation and migration via ERK signalling.

Figure 7

(a) Effect of ERK inactivation on FSTL1 knockdown-induced cellular viability under hypoxia in MTT assay. n = 3. (b) Effect of ERK inactivation on FSTL1 knockdown-induced DNA synthesis under hypoxia in BrdU assay for flow cytometer analysis. Cells in synthesis phrase (S, P4) at a cell cycle was calculated as the percent of P4/(P3 + P4 + P5). n = 3. (c) Effect of ERK inactivation on FSTL1 knockdown-induced cellular migration under hypoxia in transwell chamber. Nuclei of trans-membrane cells were stained with DAPI (blue). n = 3. Bar = 50 μm. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001. ERK = extracellular regulated kinase. P3 = G0/G1 phrase. P4 = S phase. P5 = G2 phrase. P6 = apoptosis phrase. DAPI = 4′,6-diamidino-2-phenylindole. BrdU = 5-bromo-2-deoxyuridine. SiRNA = small interfering RNA. MTT = 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.