Figure 2. Experiment I.

(A) Schematic representation of the behavioural treatments. From left to right: Alone_Ctrl–alone focal fish (red outline) administered with water; Alone_AS–alone focal fish (red filling) administered with AS; SB (O + V)_Ctrl–focal fish (green outline) administered with water and exposed simultaneously to shoal water and a shoal of 8 conspecifics, and SB (O + V)_AS–focal fish (green filling) administered with AS and exposed simultaneously to shoal water and a shoal of 8 conspecifics. Grey and red drops represent water and AS administration, respectively. (B) 3D plots representative of each behavioural treatment. Each 3D plot represents the first 5 min after AS onset for the focal fish closest to the mean in each treatment. n = 20 per treatment. Total freezing percentages presented (red circles) in each 3D plot are (from left to right): Alone_Ctrl–1.95%; Alone_AS–56.24%; SB (O + V)_Ctrl–0.00% and SB (O + V)_AS–23.32%. (C) Freezing % in baseline (Bl) vs. first 5 min after AS onset (AS). n = 20 per treatment. Mean ± SEM are shown. *p’ < 0.05; **p’ < 0.01 and ***p’ < 0.001. (D) Freezing % over the 30 min test in 10 min bins. n = 20 per treatment. Mean ± SEM are shown. *p’ < 0.05; **p’ < 0.01 and ***p’ < 0.001.