Table 1. Possible target genes and their functions for two caste-associated miRNAs isolated from female larvae of Bombus terrestris.
| MicroRNA | Gene name | Accession number | Target Gene Biological Function (Gene Ontology terms from FlyBase) |
|---|---|---|---|
| Bte-miR-6001-5p | Ecdysone-induced protein 75 (E75) | XM_003398942.2 | Antimicrobial humoral response, Ecdysis, Molting cycle, Regulation of ecdysteroid metabolic process, regulation of gene expression, regulation glucose metabolic processes, response to ecdysone |
| Ephrin receptor tyrosine kinase (EphR), transcript variant X12 | XM_012315751.1 | Melanotic encapsulation of foreign target, Mushroom body development, Peripheral nervous system development, Regulation of glucose metabolic process | |
| Vacuolar protein sorting 13B (Vps13B)-like | XM_012317130.1 | Protein targeting to the vacuole | |
| Lysine-specific demethylase (lid) | XM_003393890.2 | Chromatin organization, Gene silencing, Heterochromatin organization, Histone H3-K4 demethylation, Imaginal disc-derived wing vein specification, Oogenesis, Positive regulation of methylation-dependent chromatin silencing, Regulation of Notch signaling pathway | |
| Ferredoxin (fdxh) | XM_003393566.2 | Positive regulation of ecdysteroid biosynthetic processes, Pupariation | |
| Parvulin prolyl isomerase 1 (parv 1) | XM_003397622.2 | Epidermal growth factor receptor (Egfr) pathway | |
| Roughened (R) | XM_003397072.2 | Adherens junction assembly, Cell adhesion, Dorsal closure, Establishment of ommatidial planar polarity, Germ-line stem cell population maintenance, Hemocyte migration, Positive regulation of cell-cell adhesion, Rap protein signal transduction, Regulation of cell shape, Regulation of embryonic cell shape, Substrate-dependent cell migration, Cell extension | |
| RNA-dependent helicase p7 | XM_003397280.2 | RNA helicase | |
| Translationally controlled tumor protein (Tctp) | XM_003397970.2 | Cellular response to gamma radiation, Double-strand break repair, Intra-S DNA damage checkpoint, Mitotic G2 DNA damage checkpoint, Positive regulation of cell size, Positive regulation of histone phosphorylation, Positive regulation of multicellular organism growth | |
| No extended memory (nemy) | XM_012312185.1 | Imaginal disc-derived wing morphogenesis, Locomotory behaviour, Memory | |
| Bte-miR-6001-3p | Distal-less (DII) | XM_012315363.1 | Antennal development, determination of ventral identity, imaginal disc-derived appendage morphogenesis, imaginal disc-derived leg morphogenesis, imaginal disc-derived wing margin morphogenesis, leg disc proximal/distal pattern formation, mushroom body development, negative regulation of gene expression, olfactory behaviour, factory nerve development, positive regulation of transcription, DNA-templated, proboscis development, specification of organ identity |
| Gish casein kinase gilgamesh | XM_003393759.2 | Glial cell migration, negative regulation of actin nucleation, olfactory learning, ommatidial rotation, protein phosphorylation, regulation of endocytic recycling, regulation of establishment of planar polarity, response to mechanical stimulus, sensory perception of pain, sperm individualization, spermatogenesis, Wnt signaling pathway | |
| beta4GalNAcTA beta 1,4-N-acetylgalactosaminyltransferase A | XM_003402833.2 | Adult locomotory behaviour, glycolipid biosynthetic process, glycosphingolipid biosynthetic process, N-acetylglucosamine metabolic process, neuromuscular junction development, sperm individualization | |
| Baboon (babo) | XM_012318461.1 | Activin receptor signaling pathway, axon guidance, determination of adult lifespan, eye-antennal disc morphogenesis, imaginal disc-derived wing morphogenesis, mushroom body development, negative regulation of autophagy, neuroblast proliferation, neuron development, positive regulation of BMP signaling pathway,positive regulation of pathway-restricted SMAD protein phosphorylation, regulation of glucose metabolic process, regulation of mitotic cell, response to UV | |
| RNA-dependent helicase p72 | XM_003397280.2 | RNA helicase |
Gene ontologies from FlyBase and gene annotations and RefSeq accession numbers from the NCBI database.