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. 2017 Mar 31;7:45649. doi: 10.1038/srep45649

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HT-29/B6 monolayers were apically inoculated for 4 hours with (A,C) 10⁶ CFU HlyA⁺ E. coli-536 bacteria per filter or with (B) 50 μl of concentrated HlyA-producing E. coli-536 supernatant and simultaneous addition of divalent cations compared to untreated monolayers with (w/) or without (w/o) (A,B) zinc or (C) barium addition. Each n = 6, ****P < 0.0005, Student’s t-test.