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. 2017 Mar 31;7:45558. doi: 10.1038/srep45558

Figure 1. Depletion of Y14 induces the expression of a p53 isoform.

Figure 1

(A) HeLa cells were transfected with control or Y14-targeting siRNA. Cell lysates were subjected to immunoblotting using antibodies against p53 (DO-1 for the N-terminal region, C-19 for the C-terminal region, and pan for entire p53), Magoh, Y14, and α-tubulin. (B) Schematic diagram of human TP53 and alternative splicing. Arrows indicate primers used for p53 detection in panel C. (C) RT-PCR of p53, Bcl-x, and GAPDH transcripts in control or Y14 siRNA-transfected HeLa cells. PCR product size is indicted in parentheses. (D) Immunoblotting (IB) of p53, Y14, and α-tubulin proteins and RT-PCR of p53 and GAPDH in control or Y14 siRNA-transfected cell lines as indicated.