Figure 3. Camptothecin disrupts Y14 depletion-induced p53β expression.
(A) HeLa cells were transfected with control or Y14 siRNA followed by treatment with compounds as indicated. Immunoblotting shows p53, Y14, Magoh, and α-tubulin. Doxo, doxorubicin. (B) RT-PCR of p53, Bcl-x, and SF1 isoforms in transfectants as in panel A. (C) RT-PCR of PNN and EIF2S2 isoforms in HeLa cells treated as in panel B.
