Skip to main content
. 2017 Mar 31;7:45577. doi: 10.1038/srep45577

Figure 2. Low concentration dinaciclib induced apoptosis in human iPS cells is p53 dependent.

Figure 2

(a) iPS cells cultured on laminin E8 fragment were treated with doxorubicin (1 μM), DMSO or different concentrations of dinaciclib for 6 hrs. Whole cell lysates were then used for Western Blot analysis to measure ɣ-H2AX levels. ß-ACTIN was used as a loading control. (b) Whole cell lysates from iPS cells treated with DMSO or 6 nM dinaciclib for 6 hrs were used to examine protein levels of p53. (c) Total mRNA was extracted from iPS cells transduced with control (CTRL) or p53-specific shRNAs (KD) and was used to measure levels of knockdown using qRT-PCR. (d) Representative phase-contrast images of p53 WT and KD iPS cells treated with 6 nM dinaciclib for 24 hrs. Scale bar = 100 μm. (e) Western blot analysis of MCL-1 in iPS cells treated with DMSO or 6 nM dinaciclib for 6 hrs. (f) Western Blot of MCL-1 in p53 WT and KD iPS cells treated with DMSO or 6 nM dinaciclib for 6 hrs.