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. 2017 Mar 31;7:45535. doi: 10.1038/srep45535

Figure 2. SDS PAGE of Kl-β-Gal and its mutants in oxidizing and reducing conditions.

Figure 2

M, protein MW marker; 1, Kl-β-Gal without 2-mercaptoehtanol; 2, R116C/T270C without 2-mercaptoehtanol; 3, R116C/T270C/G818C without 2-mercaptoehtanol; 4, Kl-β-Gal with 2-mercaptoehtanol; 5, R116C/T270C with 2-mercaptoehtanol; 6, R116C/T270C/G818C with 2-mercaptoehtanol. The arrow points to the monomer form (118 KDa) and upper forms represent diverse high-order forms. The quantity of the monomer form diminishes gradually in 1, 2 and 3, whereas in 4, 5 and 6 it remains constant.