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| β-PNPG | Lactose | |||||
|---|---|---|---|---|---|---|
| Km (mM) | Vmax (U/mg) | Vmax/Km | Km (mM) | Vmax (U/mg) | Vmax/Km | |
| Kl-β-Gal | 2.15 ± 0.52 | 71.90 ± 4.67 | 33.44 | 34.31 ± 8.87 | 15.99 ± 1.48 | 0.47 |
| R116C/T270C | 2.22 ± 0.25 | 99.42 ± 3.02 | 44.78 | 38.20 ± 10.25 | 15.24 ± 1.52 | 0.40 |
| R116C/T270C/G818C | 2.47 ± 0.36 | 146.30 ± 6.11 | 59.23 | 34.82 ± 9.49 | 28.48 ± 2.80 | 0.82 |
The ± sign refers to standard error curve fit using the kinetic module of Prism 6.
