Table 2. Selection of highly accurate sequence reads (10-pass CCS reads) in 12 paired samples from 6 non-SVR cases.

Case #		Data size of total raw reads (FASTQ, Mb)	Data size of total 10-pass CCS read (FASTQ, Mb)	Base number of total 10-pass CCS read	Base number of selected 10-pass CCS reads*	# of selected 10-pass CCS reads
#1	Pre	1,803	7.7	3,884,777	1,176,880	375
	Post	197	3.8	1,643,899	682,340	218
#2	Pre	1,259	17.9	8,989,951	1,539,240	508
	Post	1,696	25.3	12,746,272	1,639,230	541
#3	Pre	955	11.1	5,518,321	598,500	285
	Post	1,245	10.8	5,440,045	615,300	293
#4	Pre	372	3.8	1,864,548	605,775	197
	Post	2,177	30.8	15,594,271	759,525	247
#16	Pre	2,194	41.0	21,887,194	611,716	196
	Post	2,387	16.6	8,355,954	955,026	306
#17	Pre	2,613	35.5	17,878,934	657,090	210
	Post	1,118	20.2	10.158,188	704,025	225

CCS, circular consensus sequence; SMRT, single molecule real time; pre, pretreatment; post, at treatment failure; *, >3000 bp-long reads aligned to genotype 1b HCV genome sequence (>2000 bp-long reads in Case#3).