Table 1.

Clinical data and ALK results

No	MCCa	MCV	age	Tumor location	Tumor size mm	ALK NGSb	ALK IHC D5F3 5A4	ALK FISHc
1	P2	pos	< 80	Right cheek		+	+ −	−
2	P3	pos	≥ 80	Posterior thigh	85	+	+ −	+
3	P4	pos	< 80	Thorax	70	+	+ −	−
4	P5	pos	< 80	Right knee	12	+	+ −
5	P9	pos	≥ 80	unknown	20	+	+ +	−
6	N10	neg	≥ 80	Right arm	50	+	+ +	+
7	N11	neg	≥ 80	Left temple	15	−	– –	−
8	P12	pos	≥ 80	Forehead	40	+	+ −	−
9	P13	pos	< 80	Right buttock	34	+	+ −	−
10	P14	pos	≥ 80	Left cheek	18	+	+ −	−
11	N17	neg	< 80	Right cheek	20	+	+ −	−
12	N18	neg	< 80	Right breast	20	+	+ −
13	N19	neg	< 80	Calf	13	+	+ −
14	N21	neg	< 80	Neck	25	+	+ −	–
15	P23	pos	< 80	Left forearm	40	+	+ −	−
16	N25	neg	≥ 80	Left back		+	+ −	+
17	P26	pos	≥ 80	Right shoulder	24	+	+ −	−
18	P28	pos	≥ 80	Left arm	75	+	−
19	N29	neg	≥ 80	Back	75	−	– –	−
20	N31	neg	< 80	Left foot	10	+	+ +	−
21	N32	neg	≥ 80	Right breast	23	−	(+) –	−
22	N33	neg	≥ 80	Front of left ear	18	−	(+) –	−
23	P34	pos	< 80	Flank	20	+	+ +	+
24	N35	neg	< 80	Upper abdomen	25	+	+ −	−
25	P36	pos	< 80	Right buttock	30	+	−
26	N37	neg	≥ 80	Right cheek	30	+	+ −	+

^aMCC tumor number. ^bCases that had high expression of ALK by NGS are marked +. ^cTumors with 3 or more dual signals in FISH are marked +. In cases N32 and N33, low proportion of tumor cells stained positive with D5F3 and thus are marked (+)