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. 2017 Mar 31;17:184. doi: 10.1186/s12906-017-1695-2

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Dose dependence of IGF-1 mediated PI3K signal pathway activation in RSC96 cell treated with AOF extract. Western blot analysis showed that the protein level of IGF-1-related PI3K signal pathway increased in a dose-dependent manner in RSC96 cells treated with AOF (20, 40, 60, 80,100, 150 and 200 μg/mL). α-tubulin was used as a loading control (a-c)