Fig. 3.

AOF extract induce the expression of PCNA and promote G₁ progression. RSC96 cells were stimulated for 24 h. a As shown by western blot analyses, AOF treatment upregulated PCNA in a dose-dependent manner. α-tubulin was used as a loading control. b Cell cycle distribution was analyzed using flow cytometry. The proportion of RSC96 Schwann cells were increased significantly in the S phase, but decreased dramatically in the G1 phase at 12 h–18 h