Table 1. IC50 values of five fractions partitioned from the methanol extract of O. fragrans.
| Materials | IC50 values (μg/ml, means ± SE) |
|||
|---|---|---|---|---|
| AGE | α–Amylase | DPPH | α–Glucosidase | |
| Methanol extract | 185.8 ± 1.6 | 275.6 ± 2.1 | 69.8 ± 2.0 | 134.5 ± 1.9 |
| Hexane fraction | 152.8 ± 1.8 | 250.2 ± 2.1 | 62.5 ± 1.4 | 120.4 ± 2.3 |
| Chloroform fraction | 110.5 ± 2.5 | 134.5 ± 1.7 | 60.7 ± 2.1 | 60.5 ± 1.6 |
| Ethyl acetate fraction | 258.8 ± 1.7 | 290.4 ± 1.9 | 75.2 ± 0.5 | 143.5 ± 2.1 |
| Butanol fraction | >500 | 325.5 ± 1.2 | 76.4 ± 0.6 | 184.3 ± 2.3 |
| Distilled water fraction | >500 | 0 | 129.8 ± 1.2 | 0 |
| Acarbose | 158.4 ± 1.4 | 75.5 ± 1.8 | ||
| Ascorbic acid | 25.5 ± 0.4 | |||
| Aminoguanidine | 54.5 ± 0.7 | |||
Acarbose was used as the positive control for α–glucosidase and α–amylase. Ascorbic acid was the positive control for DPPH. Aminoguanidine was the positive control for the inhibition of AGEs formation.