Table 4. IC50 values of isolated active constituents and their structural analogues.
| Chemicals | IC50 values (μg/ml, means ± SE) |
|||
|---|---|---|---|---|
| AGE | α–Amylase | DPPH | α–Glucosidase | |
| 4-(2,6,6-Trimethyl-2-cyclohexenyl)-3-buten-2-one | 50.5 ± 1.8 | 87.2 ± 1.3 | 23.2 ± 2.5 | 33.2 ± 1.8 |
| 4-(2,6,6-Trimethyl-1-cyclohexenyl)-3-buten-2-one | 48.4 ± 2.3 | 84.4 ± 0.8 | 20.5 ± 1.5 | 31.5 ± 2.2 |
| 9,12-Octadecadienoic acid | 15.4 ± 1.8 | 15.8 ± 2.3 | 6.8 ± 1.2 | 3.4 ± 1.5 |
| 9,12,15-Octadecatrienoic acid | 18.2 ± 2.3 | 21.2 ± 3.6 | 7.4 ± 0.9 | 4.5 ± 2.4 |
| 9-Octadecenoic acid | 18.8 ± 1.1 | 23.5 ± 1.8 | 7.6 ± 1.5 | 4.8 ± 0.9 |
| 1-Heptadecanecarboxylic acid | 34.7 ± 2.6 | 140.5 ± 2.3 | 15.6 ± 2.2 | 46.2 ± 2.5 |
| 1-Pentadecanecarboxylic acid | 40.6 ± 0.7 | 168.2 ± 1.4 | 20.8 ± 1.3 | 55.4 ± 3.2 |
| 1-Tridecanecarboxylic acid | 51.2 ± 1.8 | 225.2 ± 2.3 | 24.5 ± 1.8 | 60.4 ± 1.2 |
| Acarbose | 158.4 ± 1.4 | 75.5 ± 1.8 | ||
| Ascorbic acid | 25.5 ± 0.4 | |||
| Aminoguanidine | 54.5 ± 0.7 | |||
Acarbose served as the positive control for α–glucosidase and α–amylase. Ascorbic acid was the positive control for DPPH. Aminoguanidine was the positive control for the inhibition of AGEs formation.