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. 2017 Mar 31;13:21. doi: 10.1186/s13007-017-0172-8

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — TIPS Flowchart. Tassels were collected from replicated field plots (1) and imaged in a PVC photography booth (2) using automated gphoto2 scripts. Image analysis by TIPS software (3) removed background noise, then binarized, smoothed, and skeletonized the tassel. Each analysis step is listed in black type, while the trait measurements resulting from that step are displayed in red type. The trait measurements were returned in a .csv file along with a figure visually showing some of the measurements (4)