Table 1.
List of primary and secondary antibodies used for Western blot analysis
| Antibody | Host | Company | Catalog number | Dilution | incubation conditions |
|---|---|---|---|---|---|
| MyD88 a | rabbit | Abcam | ab2064 | 1:500 | Overnight 4 °C |
| TRIF b | rabbit | Abcam | ab13810 | 1:1000 | Overnight 4 °C |
| IL-1β | rabbit | Proteintech | 16806-1-AP | 1:500 | Overnight 4 °C |
| IBA1 | rabbit | Proteintech | 10904-1-AP | 1:500 | Overnight 4 °C |
| TNF-α | rabbit | Sino Biological | 80045-RP02 | 1:500 | Overnight 4 °C |
| GFAP | rabbit | Cell signaling technology | #12389 | 1:1000 | Overnight 4 °C |
| HMGB1 | rabbit | Cell signaling technology | #6893 | 1:1500 | Overnight 4 °C |
| ERK | rabbit | Cell signaling technology | #4695 | 1:2000 | Overnight 4 °C |
| pERK | rabbit | Cell signaling technology | #4370 | 1:1000 | Overnight 4 °C |
| NF-κB | rabbit | Cell signaling technology | #8242 | 1:1000 | Overnight 4 °C |
| pNF-κB | mouse | Cell signaling technology | #13346 | 1:1000 | Overnight 4 °C |
| β-actin | mouse | Santa Cruz Biotechnology | TA-09 | 1:1000 | Overnight 4 °C |
| Anti-rabbit IgG horseradish peroxidase (HRP) | Goat | ZSGB-BIO | ZDR-5306 | 1:3000 | 1 h RT |
| Anti-mouse IgG horseradish peroxidase (HRP) | Goat | ZSGB-BIO | ZDR-5307 | 1:3000 | 1 h RT |
a MyD88 antibody (Abcam, catalog #ab2064) was used for IHC or WB in 18 research articles (PubMed ID: 27389279, 26191134, 25446227, 26224622, 24307174, 24410883, 24477912, 24719558, 25187650, 23479602, 21278343, 20212458, 20837465, 20456021, 19553541, 17503341, 17114422, 16517734, 16461741)
b TRIF antibody (Abcam, catalog #ab13810) was used for IHC or WB in 11 research articles (PubMedID: 27389279, 26191134, 24146036, 22361049, 20511556, 19265160, 19648648, 19389833, 17503341, 17314314, 17000866)