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. 2017 Mar 31;7:45701. doi: 10.1038/srep45701

Figure 5. Compounds 5a–t exert neuroprotection in in vitro models of AD.

Figure 5

Neuroprotective effect of compounds 5a–t at 1 μM, melatonin (1 μM) and SB216763 (1 μM) against toxicity elicited by tau hyperphosphorylation (A, OA, 20 nM) or oxidative stress and energy depletion (B, R/O, 30/10 μM). SH-SY5Y cells were pre-incubated for 24 h with compounds 5a–t, melatonin or SB216763 at 1 μM. Then, treatments were removed and cells were co-incubated with each treatment and okadaic acid for 18 h or R/O for 24 h. Cell viability was measured by the MTT reduction assay. Values are expressed as mean ± SEM of five independent experiments in triplicate. ***p < 0.001; **p < 0.01 respect to toxic stimuli treated cells, considered as 0% of protection.