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. 2017 Mar 31;7:45701. doi: 10.1038/srep45701

Figure 6.

Figure 6

Nitrites (A), TNFα (B), iNOS (C) and NOX activity (E) blockade (D) and NOX2 production (F) by 5c in primary glial cultures stimulated with LPS (1 μg/mL), HO-1 expression induced by this compound after 24 h. Primary glial cells cultures were treated with compound 5c at increasing concentrations (3, 10, 30 and 60 μM) for 24 h, thereafter, treatments were removed and glial cells were co-incubated with compound 5c at increasing concentrations and LPS (1 μg/mL) for 18 h more. To measure NOX2 and HO-1 expression glial cultures were treated with compound 5c (30 μM) for 24 h. NOX activity was measured after 4 h co-treatment with LPS and compound 5c. Data are means ± SEM of four different experiments in triplicate. ###p < 0.001 compared to basal levels; ***p < 0.001; **p < 0.01; *p < 0.05 compared to LPS condition.