Figure 5.

Ibrutinib induces apoptosis of primary ALL cells. (A) Primary ALL cells cocultured with KUSA-H1 stromal cells were treated with ibrutinib (ibr) for 48 hours. Representative counter plots show a fraction of viable ALL cells as determined by DiOC₆/PI staining and flow cytometry. (B) Ibrutinib significantly reduced viability of primary ALL cells after 48 hours of treatment. Percentage of viable ALL cells was normalized to DMSO-treated control. Displayed are the mean with 95% CI of 7 ALL samples. (C) Ibrutinib inhibits Akt in sensitive ALL cells (ALL 4) but not in insensitive cells (ALL 6), as determined by western blotting. *P < .05.