Figure 4.
Retinopathy in FH W1206R mutant mice. (A) A 5-week-old male FHW/W mouse showed normal appearance of retina on funduscopy and rapid dye distribution across retinal blood vessels (<30 seconds) on fluorescein angiography. (B) A 10-week-old male FHR/R mouse exhibited retinal whitening and cotton wool spots by funduscopy and widespread nonperfusion of retinal vasculature, with dilation of the few perfused vessels by fluorescein angiography. This appearance is consistent with a central retinal artery occlusion with limited perfusion provided by a cilioretinal artery. (C) A 5-week-old FHR/R mouse showed retinal whitening by funduscopy and stenosis of vessel (white arrow) by fluorescein angiography. (D) A 10-week-old FHR/R mouse exhibited multiple hypopigmented spots on funduscopy and dye leakage (red arrows) by fluorescein angiography. (E) A 5-week-old male FHR/R mouse had detached retina in both eyes by funduscopy. (F) Time lapse analysis of the arteriovenous phase in fluorescein angiography shows delayed perfusion of retinal blood vessels in FHR/R mice (n = 21) compared with FHW/W mice (n = 10). Data are presented mean ± SD. ***P < .001 (Student t test). In panels B-E, the time label on fluorescein angiography designates the time point at which the picture was taken after dye injection. Scale bars, 200 μm.