Figure 2.

Astragaloside IV decreased LPS-induced expression of CCR5 in renal tissue during endotoxinemia. (A) Representative Western Blot image of CCR5, and the relative ratio of protein expression in each group compared to the control group; (B) The relative ratio of mRNA levels in each group compared to the control group. The protein expression of CCR5 was detected by Western Blot, and mRNA of CCR5 was detected by RT-PCR. LPS increased protein and mRNA levels of CCR5. High-dose AS-IV attenuated the increase of protein and mRNA of CCR5 in LPS-subjected animals (mean ±SEM, n=8/group). * Indicates that p<0.05 when compared to the LPS group. LPS – lipopolysaccharide; LA – low-dose AS-IV; MA – medial-dose AS-IV; HA – high-dose AS-IV; CCR5 – C-C chemokine receptor type 5.