Figure 6.

VEGF-D increases outflow facility via VEGFR-2 in enucleated mouse eyes. Cello plots showing the relative difference in C between contralateral eyes of C57BL/6 mice perfused with or without VEGF-D at 0.5 or 1.0 μg/ml. The average facility difference was 52% in response to 1.0 μg/ml VEGF-D (P = 0.004, n = 8, weighted t-test), while 0.5 μg/ml VEGF-D had no detectible effect on facility with an average difference of −8% (P = 0.70, n = 9). No facility difference was observed in response to 1.0 μg/ml VEGF-D in the presence of 1 nM Ki8751 compared to the contralateral eye perfused with Ki8751 alone (P = 0.35, n = 9), indicating that the facility-increasing effect of VEGF-D is mediated via VEGFR-2. Data points represent the relative facility difference of a treated eye with respect to its contralateral untreated eye for individual pairs. The thick white lines represent the geometric mean of the relative difference for each group. The remaining symbols are as defined in Figure 2.