Table II.
Primer | RT stem-loop primer sequence (5′-3′) | Forward primer sequence (5′-3′) | Reverse primer sequence (5′-3′) |
---|---|---|---|
miR-125b-5p | GAAAGAAGGCGAGGAGCAGATCG | CGCTCCCTGA | CGAGGAAGAA |
AGGAAGAAGACGGAAGAATGTGC | GACCCTAACT | GACGGAAGAAT | |
GTCTCGCCTTCTTTCTCACAAGT | |||
miR-324-5p | GAAAGAAGGCGAGGAGCAGATCG | CGCATCCCC | CGAGGAAGAA |
AGGAAGAAGACGGAAGAATGT | TAGGGCAT | GACGGAAGAAT | |
GCGTCTCGCCTTCTTTCACACCAAT | |||
miR-326 | GAAAGAAGGCGAGGAGCAGATCG | GCCTCTGG | CGAGGAAGAA |
AGGAAGAAGACGGAAGAATGTGCG | GCCCTTCC | GACGGAAGAAT | |
TCTCGCCTTCTTTCCTGGAGGA | |||
U6 | CGCTTCACGAATTTGCGTGTCAB | GCTTCGGCAGCACA | CGCTTCACGAA |
TATACTAAAAT | TTTGCGTGTCAT |
miR, microRNA; RT, reverse transcription.