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Illustration of the definitions of the binding free energy ΔG and ΔΔG. ΔG = GB − GU. ΔΔG = ΔGholoCaM-CaMBT − ΔGholoCaM. B and U stand for bound and unbound states of the Ca2+, respectively. ΔΔG >0 means that the CaMBT destabilizes the bound state and thus decreases the Ca2+ affinity.
