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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Neurochem Int. 2017 Feb 8;105:21–31. doi: 10.1016/j.neuint.2017.02.003

Figure 3. Prototypical S1R agonists facilitate secretion of BDNF from CCF-STTG1 cells.

Figure 3

Levels of secreted BDNF were measured using in situ ELISA. Data is normalized to the maximal response produced by stimulation of endogenous EP2 receptors. A, The S1R reference agonists 4-PPBP and (+)-SKF10047, exert a robust BDNF secretion response that is reduced by a S1R-selective dose of the S1R reference antagonist BD1063. However, BD1063 does not modify the PGE2 stimulated response mediated by EP2R. B, At a concentration that causes maximal secretion of BDNF via interactions with the EP2R, PGE2 (10 μM) is unable to displace specific bound radioligand from either S1R or S2R. [3H]-(+)-pentazocine was the radioligand used to probe the S1R in MCF-7 cells stably expressing the cloned human S1R, while [3H]-DTG was used as the radioligand to probe the S2R in untransfected MCF-7 cells.