Skip to main content
. Author manuscript; available in PMC: 2017 Sep 8.
Published in final edited form as: Nature. 2017 Mar 8;543(7645):447–451. doi: 10.1038/nature21705

Extended Data Figure 6. Binding of full-length and truncated HIF-1α and CITED2 transactivation domain peptides to 15N-TAZ1.

Extended Data Figure 6

a,c, Representative regions from superimposed 1H-15N HSQC spectra of 15N-TAZ1 bound to HIF-1α and CITED2 peptides. In a, free 15N-TAZ1 is shown in gray, 15N-TAZ1 bound to HIF 796-826 is shown in red, and 15N-TAZ1 bound to HIF 776-826 is shown in black. In c, 15N-TAZ1 is free (gray), bound to CITED 216-242 (red), and bound to CITED 216-269 (black). b, Weighted average 1H-15N chemical shift changes (Δδav) for each 15N-TAZ1 residue upon addition of HIF-1α 776-826 (black) and HIF-1α 796-826 (red). d, Weighted average 1H-15N chemical shift changes (Δδav) for each 15N-TAZ1 residue upon addition of CITED2 216-269 (black) and CITED2 216-242 (red). Weighted average 1H-15N chemical shift changes were calculated as Δδav = [(δH)2 + (δN/5)2]1/2.