Extended Data Figure 3. Monitoring HIF-1α and CITED2 competition for ¹⁵N-TAZ1 binding by NMR spectroscopy.

a, Full ¹H-¹⁵N HSQC spectra from NMR competition experiments with HIF-1α and CITED2 transactivation domain peptides. Superimposed spectra are shown for ¹⁵N-TAZ1 in the presence of one molar equivalent of HIF-1α (black), one molar equivalent of CITED2 (cyan), and one molar equivalent of both HIF-1α and CITED2 peptides (red, with fewer contours displayed for visibility). The tryptophan indole amide resonances are shown as an inset in the lower left corner. b, Detailed view of selected ¹⁵N-TAZ1 resonances. The spectral region highlighted in panel b is marked by the dotted lines on the full spectra in a. The spectra are displayed as described for a.