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. Author manuscript; available in PMC: 2017 Nov 1.
Published in final edited form as: Hum Mutat. 2016 Aug 21;37(11):1180–1189. doi: 10.1002/humu.23055

Figure 4.

Figure 4

HSJ1b deficiency does not alter Hsp70 mRNA levels but alters both MAPT and BDNF levels. A: Hsp70 mRNA expression levels are not significantly altered in wt (black column) versus mutant (solid gray column) transfected HEK293 cells (P= ns). B: MAPT (TAU) mRNA levels are significantly reduced in HSJ1b mutant transfected HEK293 cells (solid gray column) when compared with their wild type counterparts (black column) (P= 0.001).C: BDNF levels are reduced in cell lysatesof HSJ1b mutant cells (P= 0.05) but not in wtHSJ1b transfected cells (black column) (ns), which showed similar BDNF levels than non-transfected cells (ns). D: Overexpression of wild type HSJ1b (black column) did not increase the release of BDNF into the cell culture media when compared with non-transfected cells (black and white column) (ns). BNDF media release is increased in mutant HSJ1b transfected cells (solid gray column) (P = 0.03). Results are representative of two (A, B) or four (C, D) separate experiments. Values represent means ± SEM. *P≤ 0.05, **P< 0.001, ns = non-significant.