Fig 2. Hydrogen gas reduced the levels of lipid peroxidation and DNA oxidation in PC12 cells during HBO therapy.

PC12 cells in logarithmic growth phase were exposed to the experimental gases for 3 h at 1 ATA or 5 ATA. (A) The MDA levels in the culture medium as an indicator for lipid peroxidation were determined using a TBARS assay kit. (B) The 8-OH-dG levels in the culture medium as an indicator for DNA oxidation were determined using a kit. The data were analyzed using ANOVA followed by Fisher’s LSD test. *p<0.05 (n = 6), compared with the Air group, ^#p<0.05 (n = 6), compared with the HBO group, and ^&p<0.05 (n = 6), compared with the Air-H₂ group. Representative results were shown from at least three repeats.