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. 2017 Mar 31;12(3):e0174753. doi: 10.1371/journal.pone.0174753

Fig 3. Redox midpoint potential of the catalytic domains of poplar PDI-A and PDI-M.

Fig 3

A. Redox midpoint potential of PDI-A (solid line) and PDI-A K56G (dotted line). B. Redox midpoint potential of the a (solid line) and a° (dotted line) modules of PDI-M measured using the PDI-M C36/39S and PDI-M C165/168S variants respectively. The titrations were carried out using a total glutathione concentration of 2 mM in the redox buffer and with a redox equilibration time of 2 h, before labelling free protein thiols by mBBr.