Table 1. Oligonucleotides used in this study for cloning and site-directed mutagenesis experiments.
name | sequences |
---|---|
PDI-L1a for | 5’CCCCCCATGGCTGAGGATGAATCAAAGGAGTAC 3’ |
PDI-L1a rev | 5’CCCCGGATCCTCAAAGTTCATCTTTAGCTGT 3’ |
PDI-M for | 5’CCCCCCCCCATATGCTATATGGGCCTTCATCTCCT 3’ |
PDI-M rev | 5’CCCCGGATCCTTATAACTCATCCTTGCTTCC 3’ |
PDI-M C36/39S for | 5’GCACCATGGTCTGGGCACTCTAAAGCTCTC 3’ |
PDI-M C36/39S rev | 5’GAGAGCTTTAGAGTGCCCAGACCATGGTGC3’ |
PDI-M C165/168S for | 5’GCACCTTGGTCGGGTCACTCTAAGAAACTGGCT3’ |
PDI-M C165/168S rev | 5’AGCCAGTTTCTTAGAGTGACCCGACCAAGGTGC3’ |
PDI-A for | 5’CCCCCCCCCATATGGTTATAACCCTAACTCCT 3’ |
PDI-A rev | 5’CCCCGGATCCTCACAAATCTTTATCATAGCC 3’ |
PDI-A K56G for | 5’TGTGTTCCCTGGTGTGGGCATTGTAAGAATTTG 3’ |
PDI-A K56G rev | 5’CAAATTCTTACAATGCCCACACCAGGGAACACA 3’ |
Restriction sites are underlined.