Figure 2.

Vaccine-induced CD8+T cell responses in AAD mice. Groups of AAD mice (6/group) were immunized twice, three weeks apart, with MVA-PB1, MVA-M1, MVA-NP, or a three-virus combination. A group of mice received, i.n. under anesthesia, a sublethal dose (0.1 LD₅₀) of CA/09 virus. Seven days later, influenza-specific CD8+T cell responses were measured from bulk splenocytes by use of an ex vivo IFN-γ-ELISPOT assay with the indicated peptides. Bars represent the mean values ± standard deviation (SD) of triplicate cultures. The data are representative of three independent experiments that gave similar results.