Figure 9. (1) After successful invasion, tachyzoites establish a parasitophorous vacuole and initiate replication.
(2) During daughter cell formation, actin labelling is observed at the IMC of the daughter cells and at the posterior pole of the mother. (3) Once the daughter cells are fully formed, the actin signal strongly localises at the posterior end of the parasites and with the remains of the mothers IMC, as it is recycled. The first actin filamentous network and ring-like structures are visualized. (4–5) Replication continues and the filamentous network is established between the tachyzoites. The actin ring continues to localize at the residual body. (6) The filaments between the parasites and the ring break in a calcium dependent manner prior to egress. The network collapses and dots of actin are detected at the posterior end of tachyzoites. (7) Tachyzoites egressing from the vacuole leave behind an accumulation of actin in the residual body.