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. 2017 Mar 29;91(8):e02143-16. doi: 10.1128/JVI.02143-16

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FIG 2 — The interaction domain of the SARS-CoV N protein and TRIM25. (A) Anti-Flag immunoprecipitates prepared from lysates of 293T cells expressing Flag-TRIM25 and GFP-tagged full-length or truncated SARS CoV N protein were analyzed by immunoblotting with anti-Flag and anti-GFP. (B) Anti-Flag immunoprecipitates prepared from lysates of 293T cells expressing Myc-N protein and the Flag-B box/CCD or Flag-SPRY domain were analyzed by immunoblotting with anti-Flag and anti-Myc. (C) Anti-Flag immunoprecipitates prepared from lysates of 293T cells expressing the GFP-N or Flag-tagged TRIM25 domain were analyzed by immunoblotting with anti-Flag and anti-GFP.