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Virus output of rCK1023:M-G1, rCK1023:M-BJ/94, and M gene point mutants of rCK1023:M-BJ/94 H9N2 viruses from infected CEFs. (A) Multistep growth curves of H9N2 viruses from CEFs inoculated at an MOI of 0.001. (B) Single replication cycle of H9N2 viruses in CEFs inoculated at an MOI of 0.01. Virus titers were determined from supernatants collected at the indicated time points. Statistical significance was based on two-way ANOVA (*, P < 0.05; **, P < 0.01; ***, P < 0.001).
