FIG 9.

Subcellular localization of HCV proteins in the context of the D263A mutant. Electroporated cells grown on coverslips were fixed at 48 h postelectroporation and processed for immunofluorescence with antibodies against viral proteins (E1, E2, core protein [C], and NS5A). Lipid droplets were stained with BODIPY 493/503 (green). Rat anti-E2 MAb 3/11 was used for the colocalization with E1 (mouse anti-E1 MAb A4). Anti-core MAb ACAP-27 was used for the colocalization with lipid droplets (LD). The NS5A protein was labeled with anti-NS5A (9E10). Nuclei were stained with DAPI (blue). Representative confocal images of individual cells are shown with the merged images in the right column. Bar, 25 μm.