TABLE 1.
Effect of DDX3X depletion or overexpression on HSV-1 gene expression
| Gene | Protein | Value under the indicated DDX3X condition (%)a |
|||
|---|---|---|---|---|---|
| Depletion |
Overexpression |
||||
| mRNA level | Protein level | mRNA level | Protein level | ||
| RL2 | ICP0 | 72 ± 10 | 36 ± 2* | 47 ± 29 | 12 ± 9 |
| RS1 | ICP4 | 71 ± 7 | 18 ± 0.6† | 48 ± 14 | 8 ± 4* |
| UL29 | ICP8 | 65 ± 4 | 52 ± 6 | 43 ± 15 | 28 ± 5 |
| UL23 | TK | 69 ± 10 | 44 ± 14 | 58 ± 12 | 9 ± 2* |
| UL31 | pUL31 | 71 ± 7 | 39 ± 13 | 48 ± 14 | 11 ± 0.04 |
| UL48 | VP16 | 58 ± 7 | 22 ± 1* | 41 ± 10 | 1 ± 1* |
a
Significant differences between mRNA and protein levels (means ± standard deviations) for each condition in two independent experiments based on bilateral Student's t tests are indicated. *, P < 0.05; †, P < 0.001.