TABLE 2.
PCR primers
| Gene | Protein | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) |
|---|---|---|---|
| RL2 | ICP0 | CTGTCGCCTTACGTGAACAA | CATCCAGAGGCTGTTCCACT |
| RS1 | ICP4 | CGACACGGATCCACGACCC | GATCCCCCTCCCGCGCTTCGTCCG |
| UL23 | TK | GTAATGACAAGCGCCCAGAT | ATGCTGCCCATAAGGTATCG |
| UL29 | ICP8 | ACATTACGTTCACGGCCTTC | GGCCATCGACACGATAGACT |
| UL48 | VP16 | GGACGAGCTCCACTTAGACG | AGGGCATCGGTAAACATCTG |
| UL31 | pUL31 | GTGAAGACCACTCCCGTCTC | ATCGTGTTGATCTGCTGCAC |
| UL55 | gB | TTTGTGTACATGTCCCCGTTTTAC | AGAAGCCGTCGACCTGCTT |
| DDX3X | DDX3X | TGCTGGCCTAGACCTGAACT | TTGATCCACTTCCACGATCA |
| GAPDH | GAPDH | GAGTCAACGGATTTGGTCGT | TTGATTTTGGAGGGATCTCG |
| IFN-β | IFN-β | AAACTCATGAGCAGTCTGCA | AGGAGATCTTCAGTTTCGGAGG |