FIG 5.

Repression of flavivirus infection occurs through IRF3-mediated interferon signaling. (A) miR-Ct/-34a- or siRNA-transfected cells were treated with SeV for 6 h. Total RNA was analyzed for ISG56 levels as described in the text. (B) Parental and IRF3KO HeLa cells were transfected with miR-Ct/-34a, followed by infection with DENV at an MOI of 5 FFU/cell. Supernatants were collected at 48 h, and infectious virus quantified by a focus-forming assay. WT, wild type. (C) Confirmation of IRF3 knockout by Western blotting (inset) and reactivity to SeV treatment by ISG56 RT-qPCR.