FIG 2.

Binding of anti-Env antibodies to bystander cells. CEM-NKR donor cells infected with HIV-1 (NL4.3 strain) were cocultivated for 2 h with uninfected target CEM-NKR cells stained with a fluorescent dye. Cells were then stained for Gag and Env and analyzed by flow cytometry. FSC, forward scatter. (A) Gating strategy for donor and target cells and levels of Gag and Env from one representative experiment. (B) The frequency of target cells that become Gag⁺ cells varies with the extent of infection of donor cells. (C) Levels surface binding of the indicated anti-Env antibodies among Gag⁺ target cells. mGO53 was used as a control.