FIG 6.

ADCC activity of nnAbs and bNAbs on reactivated HIV-1-infected cells from patients. (A) Reactivated cells from one representative patient (patient KB51) were incubated with 5-25, PGT128, or the mGO53 isotype antibody (all at 15 μg/ml) and with heterologous NK cells. After 6 h, the percentage of Gag⁺ target cells (indicated in blue) was measured by flow cytometry. (B) Summary of ADCC observed for each antibody against reactivated CD4 T cells isolated from patients. Broadly neutralizing antibodies are color-coded in red, nonneutralizing antibodies are in blue, and the isotype control is in green. Each dot represents data for one patient, tested with the indicated Abs and NK cells isolated from 2 to 3 heterologous healthy donor cells. (C) Comparison of ADCC activities observed with nnAbs and bNAbs. Each dot represents the mean ADCC activity of each Ab, tested on cells from 6 to 8 patients. Black bars indicate the means (**, P < 0.005 by a Mann-Whitney test).